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monoclonal rabbit anti gr antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc monoclonal rabbit anti gr antibody
    Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or <t>monoclonal</t> ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm
    Monoclonal Rabbit Anti Gr Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+anti+gr/pmc13064270-124-57-61
    Average 86 stars, based on 1 article reviews
    monoclonal rabbit anti gr antibody - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Alpha-1 antitrypsin–glucocorticoid receptor axis: a new pathway in immune modulation"

    Article Title: Alpha-1 antitrypsin–glucocorticoid receptor axis: a new pathway in immune modulation

    Journal: Molecular Medicine

    doi: 10.1186/s10020-026-01470-z

    Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm
    Figure Legend Snippet: Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm

    Techniques Used: Staining, Laser-Scanning Microscopy

    Related Articles

    Incubation:

    Article Title: Glucocorticoids stimulate hypothalamic dynorphin expression accounting for stress-induced impairment of GnRH secretion during preovulatory period.
    Article Snippet: Stress-induced reproductive dysfunction is frequently associated with increased glucocorticoid (GC) levels responsible for suppressed GnRH/LH secretion and impaired ovulation.. Besides the major role of the hypothalamic kisspeptin system, other key regulators may be involved in such regulatory mechanisms.. Herein, we identify dynorphin as a novel transcriptional target of GC.

    Article Title: Glucocorticoid receptor signaling in astrocytes is required for aversive memory formation
    Article Snippet: Serial 40-μm-thick coronal sections from CTRL and GR astroKO mice obtained with vibratome were processed in parallel. .. Free-floating sections were rinsed in PBS, incubated with 10% normal goat serum in PBS with 0.2% Triton X−100 (PBS-Tx) for 90 min at room temperature (RT) and incubated overnight at RT with antibodies: polyclonal anti-S100β (1:250, 287004, Synaptic Systems) or a monoclonal anti-NeuN (1:200, MAB377, Merck Millipore) and a monoclonal anti-GR (1:100, 12041 S, Cell Signalling) diluted in PBS-Tx containing 1% normal serum. .. The next day, sections were washed in PBS and incubated for 90 min at RT with fluorophore-conjugated secondary antibodies (A11073 and A11036, ThermoFisher), diluted 1:250 in PBS-Tx containing 1% normal serum.

    Article Title: Acute stress responsiveness of the neurotrophin BDNF in the rat hippocampus is modulated by chronic treatment with the antidepressant duloxetine.
    Article Snippet: Equal amounts of protein were run under reducing conditions on SDS–polyacrylamide gels (14% SDS–PAGE for BDNF and 8% SDS–PAGE for glucocorticoid receptor (GR), a-calcium/calmodulin-dependent protein kinase II (aCaMKII), CREB, methyl CpG-binding protein 2 (MeCP2), mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)) and then electrophoretically transferred onto PVDF (for BDNF) or nitrocellulose (for the other proteins) membranes (Bio-Rad). .. The blots were blocked with 10% nonfat dry milk and then incubated with the following primary antibodies: anti-BDNF polyclonal antibody (1 : 1000, 41C, overnight; Santa Cruz Biotechnology, Santa Cruz, CA, USA) able to recognize both the mature form of the neurotrophin (mBDNF; 14 kDa) and its precursor (proBDNF; 32 kDa); monoclonal anti-GR (Affinity Bioreagent, Golden, CO, USA; dilution 1 : 2500); monoclonal anti-Thr286-phospho-a-CaMKII (Affinity Bioreagent; dilution 1 : 2500); monoclonal anti-a-CaMKII (Chemicon International Inc., Temecula, CA, USA; dilution 1 : 2500); monoclonal anti-Ser133-phospho-CREB (Cell Signaling Technology, Danvers, MA, USA; dilution 1 : 1000); monoclonal anti-CREB (Cell Signaling Technology; dilution 1 : 1000); monoclonal anti-Ser217/221 phospho-MEK (Santa Cruz Biotechnology; dilution 1 : 500); monoclonal anti-MEK (Santa Cruz Biotechnology; dilution 1 : 5000); monoclonal anti-Ser421-phospho-MeCP2 and monoclonal anti-MeCP2 (both generous gifts of Professor ME Greenberg; dilution 1 : 4000 and 1 : 2000, respectively). .. Membranes were then incubated for 1 h at room temperature with a peroxidaseconjugated antirabbit IgG (1 : 5000 for BDNF and 1 : 2000 for the other proteins) and immunocomplexes were visualized by chemiluminescence using the ECL Western Blotting kit (Amersham Life Science, Milan, Italy).

    Blocking Assay:

    Article Title: Glucocorticoids stimulate hypothalamic dynorphin expression accounting for stress-induced impairment of GnRH secretion during preovulatory period.
    Article Snippet: Stress-induced reproductive dysfunction is frequently associated with increased glucocorticoid (GC) levels responsible for suppressed GnRH/LH secretion and impaired ovulation.. Besides the major role of the hypothalamic kisspeptin system, other key regulators may be involved in such regulatory mechanisms.. Herein, we identify dynorphin as a novel transcriptional target of GC.

    Saline:

    Article Title: Glucocorticoids stimulate hypothalamic dynorphin expression accounting for stress-induced impairment of GnRH secretion during preovulatory period.
    Article Snippet: Stress-induced reproductive dysfunction is frequently associated with increased glucocorticoid (GC) levels responsible for suppressed GnRH/LH secretion and impaired ovulation.. Besides the major role of the hypothalamic kisspeptin system, other key regulators may be involved in such regulatory mechanisms.. Herein, we identify dynorphin as a novel transcriptional target of GC.



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    Image Search Results


    Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm

    Journal: Molecular Medicine

    Article Title: Alpha-1 antitrypsin–glucocorticoid receptor axis: a new pathway in immune modulation

    doi: 10.1186/s10020-026-01470-z

    Figure Lengend Snippet: Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm

    Article Snippet: Adherent cells were fixed with 4% paraformaldehyde (PFA) for 10 min at 37 °C, permeabilized with 0.05% Triton-X100 for 5 min and blocked with 5% BSA in PBS for 1 h. AAT was stained with monoclonal mouse-anti AAT antibody (Proteintech, dilution 1:200) and GR was stained with polyclonal rabbit anti-GR antibody (Thermo Fisher Scientific, dilution 1:100) or monoclonal rabbit anti-GR antibody (Cell Signaling, dilution 1:200).

    Techniques: Staining, Laser-Scanning Microscopy